Geometric Changes of Transverse Tubules in Rabbit Cardiac Myocytes during Contraction
نویسندگان
چکیده
منابع مشابه
Ionic diffusion in transverse tubules of cardiac ventricular myocytes.
We have estimated the rate of diffusion of calcium ions in the transverse tubules of isolated cardiocytes by recording changes in peak calcium current ( I Ca) caused by rapid changes of the extracellular calcium concentration ([Ca]o) at various intervals just preceding activation of I Ca. Isolated ventricular cells of guinea pig heart and atrial cells from rabbit heart were voltage-clamped (who...
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or degraded and are replaced by actin and av-actinin-positive, myosin-negative structures that resemble myofibrils but lack thick filaments. Such structures are termed minute myofibrils. The length of the sarcomeres in these altered myofibrils is significantly less than length values obtained from freshly isolated heart cells or from contracting myocytes. A number of high density cultures devel...
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The expression and subcellular distribution of the dystrophin-glycoprotein complex and laminin were examined in cardiac muscle by immunoblot and immunofluorescence analysis of rabbit and sheep papillary muscle. The five dystrophin-associated proteins (DAPs), 156-DAG, 59-DAP, 50-DAG, 43-DAG, and 35-DAG, were identified in rabbit ventricular muscle and found to codistribute with dystrophin in bot...
متن کاملSubcellular [Ca2+]i gradients during excitation-contraction coupling in newborn rabbit ventricular myocytes.
The central role of T-tubule and sarcoplasmic reticulum (SR) diadic junctions in excitation-contraction (EC) coupling in adult (AD) ventricular myocytes suggests that their absence in newborn (NB) cells may manifest as an altered EC coupling phenotype. We used confocal microscopy to compare fluo-3 [Ca2+]i transients in the subsarcolemmal space and cell center of field-stimulated NB and AD rabbi...
متن کاملNeonatal, and Adult Rabbit Cardiac Myocytes
Measurement of transsarcolemmal voltage-gated Ca2' current ('Ca) in myocytes isolated from immature rabbit heart has demonstrated an unexpectedly low level of Ca2` channel activity. We have characterized the kinetic properties of lCa in acutely isolated 21-day fetal, 1-5-day-old neonatal, and adult cardiac myocytes by the whole-cell voltage-clamp technique. The membrane potential for half-maxim...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.1947